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The adult body contains approximately 1kg of calcium. The majority of this calcium is found in the bone calcium hydroxyapatite. Calcium in the skeleton is critical for the strength and structure of bones but have also an important role in the maintenance of calcium homeostasis through bone formation and resorption. Calcium metabolism is regulated by parathyroid hormone (PTH), vitamin D, phosphate and magnesium (Goldstein, 1990; Ross, 2011). This element plays numerous roles in biological function such as muscle contraction, intra- and inters- cellular signaling and nerve impulse transmission. Hypocalcemia and Hypercalcemia can be detected through measurement of serum calcium and are indicative of several pathologies (Goldstein, 1990; Peacock, 2010).

The validated and used assay in the laboratory is an automated colorimetric assay for the measurement of Calcium in urine or in serum.


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